Bergamot essential oil differentially modulates intracellular Ca2+ levels in vascular endothelial and smooth muscle cells: a new finding seen with fura-2

You JH, Kang P, Min SS, Seol GH
Journal of Cardiovascular Pharmacology, 2013

ABSTRACT:

Abstract: In this study, we compared the effect of the essential oil of Citrus bergamia Risso [bergamot, bergamot essential oil (BEO)] on the intracellular Ca²⁺ levels in vascular endothelial (EA) and mouse vascular smooth muscle (MOVAS) cells, using the fura-2 fluorescence technique. BEO caused an initial transient increase in intracellular Ca²⁺concentration ([Ca²⁺]_i) in EA cells, followed by a decrease, whereas it induced a sustained increase in [Ca²⁺]_i in MOVAS cells. Linalyl acetate (LA) as a major component of BEO-induced [Ca²⁺]_i mobilization was similar to BEO in EA cells. The increase of [Ca²⁺]_i by LA was higher in EA cells than in MOVAS cells. [Ca²⁺]_i rise induced by extracellular Ca²⁺application was significantly blocked by BEO or LA in EA cells but not in MOVAS cells, suggesting that BEO and LA block Ca²⁺ influx in EA cells. The present results suggest that BEO and LA differentially modulate intracellular Ca²⁺ levels in vascular endothelial and smooth muscle cells. In addition, blockade of Ca²⁺ influx by BEO and LA in EA cells may explain the protective effects of BEO on endothelial dysfunction associated with cardiovascular disease.

CITATION:

You JH, Kang P, Min SS, Et Al. Bergamot essential oil differentially modulates intracellular Ca2+ levels in vascular endothelial and smooth muscle cells: a new finding seen with fura-2. J Cardiovasc Pharmacol. 2013;61(4):324-328.

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