Mahmoudvand H, Mahmoudvand H, Oliaee RT, Kareshk AT, Mirbadie SR, Aflatoonian MR
Pharmacognosy Magazine, 2017
BACKGROUND:
This study investigates the scolicidal effects of Cinnamomum zeylanicum essential oilagainst the protoscoleces of hydatid cysts and its toxicity in the mice model.
MATERIALS AND METHODS:
Gas chromatography/mass spectroscopy analyses were used to identify the constituents of essential oil. Protoscoleces were treated with different concentrations of the essential oil(6.25-100 µL/mL) in each test tube for 5-30 min. The viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Forty-eight male NMRI mice were also used to determine the toxicity of C. zeylanicum essential oil (0.5-4 mL/kg).
RESULTS:
The main components were found to be cinnamaldehyde (91.8%), ρ metoxicinamate (1.57%), and α pinene (1.25%). Findings indicate that C. zeylanicum essential oil with the concentrations of 100 and 50 µL/mL killed 100% of protoscoleces after 5 min of exposure. Also, the lower concentrations of C. zeylanicum essential oil motivated a late protoscolicidal effect. The LD50 value of intraperitoneal injection of C. zeylanicum essential oil was 2.07 mL/kg body weight after 48 h, and the maximum nonfatal dose was 1.52 mL/kg body weight. The results also showed that there was no significant toxicity following oral administration of C. zeylanicum essential oil for 2 weeks.
CONCLUSION:
The results exhibited the favorable scolicidal activity of C. zeylanicum, which could be applied as a natural scolicidal agent in hydatid cyst surgery.
SUMMARY:
We evaluated the efficacy of Cinnamomum zeylanicum essential oil against hydatid cyst protoscoleces. The viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining)Forty-eight male NMRI mice were also used to determine the toxicity of C. zeylanicum essential oilC. zeylanicum with potent scolicidal activity could be applied as a natural scolicidal agent in surgery. Abbreviations used: GC/MS: Gas chromatography/mass spectrometry analysis; CE: Cystic echinococcosis; LD50: Lethal dose 50%; I.p: Intraperitoneally.
CITATION:
Mahmoudvand H, Mahmoudvand H, Oliaee RT et al. In vitro Protoscolicidal Effects of Cinnamomum zeylanicum Essential Oil and Its Toxicity in Mice. Pharmacogn Mag. 2017 Oct;13(Suppl 3):S652-S657. doi: 10.4103/pm.pm_280_16. Epub 2017 Oct 11.
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